Val of extremity soft-tissue sarcoma: results beyond the staging syste…
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Val of extremity soft-tissue sarcoma: results beyond the staging system. Cancer 2011, 117:1055-1060. 27. Heck RK Jr, Peabody TD, Simon MA: Staging of primary malignancies of bone. CA Cancer J Clin 2006, 56:366-375. Pre-publication history The pre-publication history for this paper can be accessed here: http://www.biomedcentral.com/1471-2474/12/250/prepubdoi:10.1186/1471-2474-12-250 Cite this article as: Morii et al.: D-dimer levels as a prognostic factor for determining oncological outcomes 3'-Oxydipropionic Acid) ca. 30% in Water ethyl 1-(4-methoxyphenyl)-7-oxo-6-(4-(2-oxopiperidin-1-yl)phenyl)-4 musculoskeletal sarcoma. BMC Musculoskeletal Disorders 2011 12:250.
Vordemvenne et al. BMC Musculoskeletal Disorders 2011, 12:263 http://www.biomedcentral.com/1471-2474/12/RESEARCH ARTICLEOpen AccessCooperative effects in differentiation and proliferation between PDGF-BB and matrix derived synthetic peptides in human osteoblastsThomas Vordemvenne1*, J gen RJ Paletta2, Rene Tert-butyl 2-(chloromethyl)pyrrolidine-1-carboxylate Hartensuer1, Thomas Pap3, Michael J Raschke1 and Sabine OchmanAbstractBackground: Enhancing osteogenic capabilities of bone matrix for the treatment of fractures and segmental defects using growth factors is an active area of research. Recently, synthetic peptides like AC- 100, TP508 or p-15 corresponding to biologically active sequences of matrix proteins have been proven to stimulate bone formation. The platelet-derived growth factor (PDGF) BB has been PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/18218841 identified as an important paracrine factor in early bone healing. We hypothesized that the combined use of PDGF-BB with synthetic peptides could result in an increase in proliferation and calcification of osteoblast-like cells. Methods: Osteoblast-like cell cultures were treated with PDGF and synthetic peptides, singly and as combinations, and compared to non-treated control cell cultures. The cultures were evaluated at days 2, 5, and 10 in terms of cell proliferation, calcification and gene expression of alkaline phosphate, collagen I and osteocalcin. Results: Experimental findings revealed that the addition of PDGF, p-15 and TP508 and combinations of PDGF/AC-100, PDGF/p-15 and PDGF/TP508 resulted in an increase in proliferating osteoblasts, especially in the first 5 days of cultivation. Proliferation did not significantly differ between single factors and factor combinations (p > 0.05). The onset of calcification in osteoblasts occurred earlier and was more distinct compared to the corresponding control or PDGF stimulation alone. Significant difference was found for the combined use of PDGF/p-15 and PDGF/AC-100 (p < 0.05). tert-Butyl (7-bromoheptyl)carbamate Conclusions: Our findings indicate that PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/10714611 PDGF exhibits cooperative effects with synthetic peptides in differentiation and proliferation. These cooperative effects cause a significant early calcification of osteoblast-like cells (p < 0.05). We suggest the combination of synthetic peptides and PDGF as a potential clinical approach for accelerating bone healing or coating osteosynthesis materials.Background Enhancing the osteogenic capabilities of bone matrix for the treatment of fractures and segmental bone defects is an active area of research. It is well-known that the use of oligopeptides or growth factors promotes osteogenic cell proliferation and differentiation in vitro and in vivo [1-4]. There are many different pathways in research to address the acceleration of fracture healing. The platelet-derived growth factor (PDGF), a paracrine factor in fracture hematoma, has proven its efficacy in* Correspondence: thomas.vordemvenne@ukmuenster.de 1 Department of Trauma, Hand and Reconstructive Surgery, Universit.
Vordemvenne et al. BMC Musculoskeletal Disorders 2011, 12:263 http://www.biomedcentral.com/1471-2474/12/RESEARCH ARTICLEOpen AccessCooperative effects in differentiation and proliferation between PDGF-BB and matrix derived synthetic peptides in human osteoblastsThomas Vordemvenne1*, J gen RJ Paletta2, Rene Tert-butyl 2-(chloromethyl)pyrrolidine-1-carboxylate Hartensuer1, Thomas Pap3, Michael J Raschke1 and Sabine OchmanAbstractBackground: Enhancing osteogenic capabilities of bone matrix for the treatment of fractures and segmental defects using growth factors is an active area of research. Recently, synthetic peptides like AC- 100, TP508 or p-15 corresponding to biologically active sequences of matrix proteins have been proven to stimulate bone formation. The platelet-derived growth factor (PDGF) BB has been PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/18218841 identified as an important paracrine factor in early bone healing. We hypothesized that the combined use of PDGF-BB with synthetic peptides could result in an increase in proliferation and calcification of osteoblast-like cells. Methods: Osteoblast-like cell cultures were treated with PDGF and synthetic peptides, singly and as combinations, and compared to non-treated control cell cultures. The cultures were evaluated at days 2, 5, and 10 in terms of cell proliferation, calcification and gene expression of alkaline phosphate, collagen I and osteocalcin. Results: Experimental findings revealed that the addition of PDGF, p-15 and TP508 and combinations of PDGF/AC-100, PDGF/p-15 and PDGF/TP508 resulted in an increase in proliferating osteoblasts, especially in the first 5 days of cultivation. Proliferation did not significantly differ between single factors and factor combinations (p > 0.05). The onset of calcification in osteoblasts occurred earlier and was more distinct compared to the corresponding control or PDGF stimulation alone. Significant difference was found for the combined use of PDGF/p-15 and PDGF/AC-100 (p < 0.05). tert-Butyl (7-bromoheptyl)carbamate Conclusions: Our findings indicate that PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/10714611 PDGF exhibits cooperative effects with synthetic peptides in differentiation and proliferation. These cooperative effects cause a significant early calcification of osteoblast-like cells (p < 0.05). We suggest the combination of synthetic peptides and PDGF as a potential clinical approach for accelerating bone healing or coating osteosynthesis materials.Background Enhancing the osteogenic capabilities of bone matrix for the treatment of fractures and segmental bone defects is an active area of research. It is well-known that the use of oligopeptides or growth factors promotes osteogenic cell proliferation and differentiation in vitro and in vivo [1-4]. There are many different pathways in research to address the acceleration of fracture healing. The platelet-derived growth factor (PDGF), a paracrine factor in fracture hematoma, has proven its efficacy in* Correspondence: thomas.vordemvenne@ukmuenster.de 1 Department of Trauma, Hand and Reconstructive Surgery, Universit.
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